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AbXtract™ from Specifica

Next Generation Sequencing (NGS)-based antibody discovery now is available in the Orion® molecular design platform. These capabilities are part of AbXtract™, which is the first module available in Orion's new Antibody Discovery Suite. AbXtract was developed in partnership with Specifica. Founded by Andrew Bradbury, PhD, an innovator in novel antibody technologies, Specifica is a leader in antibody libraries design and discovery.

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Use AbXtract™ in Orion® to identify antibodies within the same functional cluster that have reduced number of liabilities.
Use AbXtract™ in Orion® to identify antibodies within the same functional cluster that have reduced number of liabilities.

Compared to conventional colony screening methods, NGS screening with AbXtract helps teams:

  • Uncover more leads. Increase the number of clonotype leads five- to ten-fold compared to random colony screening
  • Increase sequence diversity and cluster representation. Explore the entire sequence diversity within selected populations, even rare clones that are typically missed by low-throughput methods that favor more abundant antibodies
  • Prioritize promising leads. Tie in known data, and even low-throughput assay data, to prioritize leads with the most favorable developability and biophysical profiles
  • Minimize costs. Achieve high throughput for a fraction of the cost of conventional assay runs
  • Equip the entire discovery team. Automated workflows for novice users, while allowing expert users to fully configure their settings

From Millions of Sequences to a Select Few

AbXtract has the cloud-based compute power to process tens of millions of sequences into meaningful information. Its sophisticated machine-learning algorithms and workflows help antibody engineers and bioinformaticians parse through this vast amount of data to characterize sequences and extract sequence- and functional-based features. Once promising leads are identified, visual models can further aid in decision-making.

User Friendly - Get Leads from Your Data in Three Guided Steps

Use AbXtract™ in Orion® to identify antibodies within the same functional cluster that have reduced number of liabilities.
Use AbXtract™ in Orion® to identify antibodies within the same functional cluster that have reduced number of liabilities.

Additional Resources

  1. Erasmus, MF, Fortunato, F., Leal-Lopes, C., D’Angelo, S., Teixeira, A., Spector, L., Perea, K., Soerensen, J., Dovner, M., A., Choudhary, A., Honnen, W., Calianese, D., Pinter, A., Bradbury, ARM. Diverse Panel of High Affinity SARS-CoV-2 Leads with Varying Specificity Derived from Long-Read Next-Generation Sequencing Pipeline. To Be Submitted (November, 2021).
  2. Ferrara, F, Erasmus, M.F., D’Angelo, S., Leal-Lopes, C., Teixeira, A., Choudhary, A., Honnen, W., Calianese, D., Huang, D., Burton, D., Pinter, A., Bradbury, A.R.M. A pandemic-enabled comparison of antibody discovery platforms: a naïve library directly delivering antibodies as potent as immune sources. Nature Comm. In press (2021).
  3. Teixeira, A., Erasmus, M.F., D’Angelo, S., Naranjo, L., Ferrara, F., Leal-Lopes, CC., Durrant, O., Galmiche, C., Morelli, A., Scott-Tucker, A., and Bradbury, A.R.M. Drug-like antibodies with high affinity, diversity and developability straight from next-generation antibody libraries. MAbs vol 13, 1 (2021).
  4. Erasmus, M.F., D’Angelo, S., Ferrara, F., Naranjo, L., Teixeira, A., Buonpane, R., Stewart, S.M., Nastri, H.G., and Bradbury, A.R.M. A single donor is sufficient to produce a highly functional in vitro antibody library. Commun Biol 4, 350 (2021).
  5. Bradbury, A. R. M., Erasmus, M. F. & Teixeira, A. Antibody libraries with maximized antibody developability characteristics. US patent (2020).

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